Graduate Study

We take graduate students through the following programs:

Zebrafish as a model for hearing loss

Understanding hair cell development, death and regeneration

Research

Publications

People

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Research

Hair cells of the inner ear act as mechanosensors, converting mechanical stimuli to electrical signals transmitted to the brain. Hair cells of the auditory organs respond to sound stimuli for hearing perception; those in the vestibular organs respond to gravity and head movements for perception of balance.

Hair cells are called such because they have actin-rich protrusions, stereocilia, at their apical end. Displacement of stereocilia opens ion channels resulting in depolarization and release of transmitter from synapses at the basal end of the cell to terminals of innervating afferent nerves.

Damage and loss of hair cells are leading causes of hearing and balance disorders, affecting over 40 million people in the US. Hair cells are susceptible to environmental insults, including noise, chemical exposure and accumulated damage during aging. Hair cell loss in humans is irreversible.

Zebrafish lateral line hair cells share many properties with those of the inner ear, and are sensitive to the same types of damaging agents as human cells. However they are able to regenerate hair cells throughout life. The location of the lateral line on the surface of the body allows access for visualization and manipulation.

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Select Publications

Nechiporuk, A. and Raible, D.W. (2008). Fgf-dependent primordium organization drives lateral line segmentation in zebrafish. Science, 320:1774-1777.

Owens, K.N., Santos, F., Roberts, B., Linbo, T., Knisely, A.J., Simon, J.A., Rubel, E.W and Raible, D.W. (2008). Identification of genetic and chemical modulators of zebrafish mechanosensory hair cell death. PLoS Genetics, 4: e1000020. PMCID: PMC2265478

Ma, E.Y., Rubel, E.W and Raible, D.W. (2008). Notch signaling regulates the extent of hair cell regeneration in the zebrafish lateral line. J. Neurosci., 28: 2261-2273.

Suli, A., Watson, G.M., Rubel, E.W., Raible, D.W. (2012). Rheotaxis in larval zebrafish is mediated by lateral line mechanosensory hair cells. PLoS One, 7:e29727. PMCID:PMC3281009

Hailey, D.W., Roberts, B., Owens, K.N., Stewart, A.K., Linbo, T., Pujol, R., Alper, S.L., Rubel, E.W, Raible, D.W. (2012). Loss Slc4a1b Chloride/Bicarbonate Exchanger Function Protects Mechanosensory Hair Cells from Aminoglycoside Damage in the Zebrafish Mutant persephone. PLoS Genet 8(10): e1002971. PMCID: PMC3469417

Mackenzie, S.M. and Raible, D.W. (2012). Proliferative regeneration of zebrafish lateral line hair cells after different ototoxic insults. PLoS One, 7: e47257. PMCID: PMC3475690

Esterberg, R., Hailey, D.W., Coffin, A.B., Raible, D.W. and Rubel, E.W. (2013). Disruption of intracellular calcium is integral to aminoglycoside-induced hair cell death. J Neurosci., 33: 7513-7525. PMCID: PMC3703319

Coffin, A.B., Rubel, E.W. and Raible, D.W. (2013). Bax, Bcl2, and p53 Differentially Regulate Neomycin- and Gentamicin-Induced Hair Cell Death in the Zebrafish Lateral Line JARO,14: 645-659. PMCID: PMC3767879

Suli, A., Guler, A.D., Raible D.W. and Kimelman, D. (2014). Targeted gene expression system using the Tryptophan Repressor in zebrafish shows no silencing in subsequent generations. Development, 141:1167-1174. PMCID: PMC3929415

Stawicki, T.M., Owens, K.N., Linbo, T., Reinhart, K.E., Rubel, EW, Raible, D.W. (2014). The zebrafish merovingian mutant reveals a role for pH regulation in hair cell toxicity and function. Disease Models Mech., 7:847-56.

Esterberg, R., Hailey, D.W., Rubel, EW, and Raible, D.W. (2014). ER-mitochondrial calcium flow underlies vulnerability of mechanosensory hair cells to damage. J. Neurosci., 34:9703-9719. PMCID: PMC4099547

Thomas, E.D., Cruz, I.A., Hailey, D.W., and Raible D.W. (2015). There and back again: Development and regeneration of the zebrafish lateral line system. Wiley Interdiscip Rev Dev Biol. 4:1-16. PMCID: PMC4268111

Cruz, I.A., Kappedal, R., Mackenzie, S.M., Hailey, D.W., Hoffman, T.L., Schilling, T.F. and Raible, D.W. (2015). Robust regeneration of adult zebrafish lateral line hair cells reflects continued precursor pool maintenance. Dev. Biol., 402: 229-238. PMCID: PMC4450121

Other publications

Lab Members

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David Raible

PI

Tor Linbo

Research Scientist

Tammy Stawicki

Postdoc

Joy Sebe

Postdoc

Heather Stickney

Postdoc

Ivan Cruz

Postdoc

Eric Thomas

Grad Student

Sarah Pickett

Grad Student

Former lab members

Graduate Students

Nick Coley
Ivan Cruz
Kevin Curran
Kelly Grant
Will Henry
Jessica Lewis
Eva Ma
Scott Mackenzie
Sarah Malmquist
Hillary McGraw
Andrew Prendergast
Jared Ragland
Josette Ungos

Resident, UC Davis
Postdoc, UW
Assistant Professor, USD
Assistant Professor, Gannon University
Assistant Attorney General, WA State
IP Attorney, Quarles and Brady
Research Scientist, UW
Editor, Inside Flyer
Lecturer, SUNY Stonybrook
Postdoc, OHSU
Postdoc, INSERM
Policy Director, Software Alliance

Postdoctoral Fellows

Alexandra Abramsson
Heather Brignull
Alli Coffin
Cynthia Cooper
Richard Dorsky
Robert Esterberg
Dale Hailey
James Lister
Alex Nechiporuk
Kelly Owens
Cat Pittack
Iain Shepherd
Corey Snelson
Arminda Suli

Researcher, University of Gothenburg
Scientific Communications
Assistant Professor, WSU Vancouver
Associate Professor, WSU Vancouver
Professor, University of Utah
Scientist, Novartis
Director, Garvey Imaging Center, ISCRM
Assistant Professor, VCU
Associate Professor, OHSU
Lecturer, UW
Associate Professor, Bastyr
Associate Professor, Emory
King County Public Health
Assistant Professor, BYU

Contact

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David Raible
draible@uw.edu
206.616.1048

Lab Phone: 206.616.7467
Address:
University of Washington
Department of Biological Structure
Heath Sciences H-501
1959 NE Pacific St.
Seattle, WA 98195-7420

© Raible Lab 2016